High concordance between next-generation sequencing and single-nucleotide polymorphism array in preimplantation genetic testing for aneuploidy

Background: This study aimed to compare the use of next-generation sequencing (NGS) and single-nucleotide polymorphism (SNP) array in preimplantation genetic testing for aneuploidy (PGT-A) same blastocyst. Methods: We performed a retrospective on 67 embryos (from 23 couples), where PGT-A was carried out. A trophectoderm (TE) biopsy blastocyst, 24-chromosomal ploidy status analyzed. Initially, 28 blastocysts with unknown were analyzed using both NGS SNP array. Thereafter, 39 euploidy detected via re-analyzed Results: In first stage, concordance rate 92.9% (26/28). Among blastocysts, 16 abnormal, 12 euploid when NGS. abnormal two showed mosaicisms but found be second 100% (39/39) analyzing normal blastocysts. After single blastocyst transfer 29 frozen embryo cycles, clinical pregnancy 75.9% (22/29), ongoing 69.0% (20/29), live birth (20/29). Nineteen couples (20 babies) had healthy babies. Their prenatal diagnosis results karyotype analysis after delivery concordant PGT results. Two cycles miscarried, abortion villus exhibited euploidy. Conclusions: There high between TE combined an efficient strategy identify suitability transfer.